effect of zinc nano-complex on bull semen quality and pregnancy outcome
نویسندگان
چکیده
this study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and pregnancy outcome after freeze-thawing process. ejaculates were collected from four holstein bulls, twice a week during four weeks. semen samples diluted with bioxcell extender containing zero (control), 10-6, 10-5, 10-4, 10-3 and 10-2 m of zinc nano-complex. the samples were then freezed-thawed after 72 hours. motility of sperm was analyzed using computer assisted sperm analysis. the highest and lowest concentrations of zinc nano-complex along with control group were analyzed for the dna fragmentation, malondialdehyde (mda) concentration, mitochondrial activity, and pregnancy outcomes. the proportion of the total and progressive motile spermatozoa and the percentage of spermatozoa with fragmented dna were not different among the groups. however, the groups containing 10-6 and 10-2 m zinc nano-complex had a lower level of mda concentration and higher activity of mitochondria. moreover, the pregnancy outcome was not affected by the treatment with zinc nano-complex. in conclusion, our data revealed that supplementation of bull semen bioxcell extender with zinc nano-complex can decrease the level of mda and improve mitochondrial activity, although such an improvement was not reflected in results of fertility rates.
منابع مشابه
effect of zinc nano-complex on bull semen quality after freeze-thawing process
the effect of zinc nano-particles (zn- nano- complex) on bull sperm quality after freeze-thawing process studied. ejaculates collected from four holstein bulls twice a week. on the day of semen collection, four ejaculates were pooled and diluted with bioxcell extender containing 0, 10-6, 10-5, 10-4, 10-3, 10-2 molar of zinc nano-complex and frozen. after thawing, sperm motility and motion param...
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چکیده ندارد.
15 صفحه اولPmn-22: The Effect of Zinc on Semen Quality
Background: Zinc in human semen seems to play an important role in the physiology of spermatozoa. Zinc deficiency leads to gonadal dysfunction, decreases testicular weight, and causes shrinkage of seminiferous tubules. The adult human body contains 1-3 g of zinc, about 0.1% of which is replenished daily. Excessively high amounts of zinc has been reported in human seminal plasma by many authors,...
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عنوان ژورنال:
تولیدات دامیجلد ۱۸، شماره ۱، صفحات ۱۷۳-۱۸۱
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